BASU BACTERIA Mac OS

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Do you want to install macOS on Linux using Virtual Machine? The Sosumi could be an easy option, though there is no 100 percent guarantee it will work.

OS: 10.12+ Sierra (64 bit) Processor: 3.0 GHz Quad Core Processor; Memory: 2 GB RAM; Graphics: NVIDIA GeForce GT 650M (1GB GDDR5) / Intel Iris graphics or greater; Storage: 1 GB available space; Additional Notes: Unsupported video chipsets: Intel HD Graphics 3000, Intel GMA X3100, Intel GMA 950. This game is not supported on volumes formatted as Mac OS Extended Journaled (Case Sensitive). Only bacteria displaying fluorescence from both DFHBI-1T and YO3-biotin were selected for. EC 50 was calculated in GraphPad Prism 7.0b for Mac OS X. Reetobrata Basu & John J.

What is Sosumi?

To install macOS on Linux using Qemu virtual machine, the developer Alan Pope (popey) has created a bundled snap package known as Sosumi. It is easy to install and works on Ubuntu, Fedora, Debian, and other major Linux distributions. Here is the Github page and source of this snap package. Or directly visit the Snap page of Sosumi.

After the installation of the Sosumi packages, the Clover bootloader will pop up and boot to the macOS recovery system. One thing that we need to note down is the Sosumi itself doesn't contain macOS instead when we fire up snap packages based macOS-Simple-KVM virtual machine to install macOS Catalina desktop it downloads an installer image for the same.

The VM created by this snap package will have a fixed resolution window, initial launch with 2GB of memory, bundles qemu-virgil, which includes virtio-vga, a paravirtual 3D graphics driver.

I tried to use it on Ubuntu 18.04 LTS and faced a couple of issues. Let me take you through the steps of macOS installation on Linux using Sosumi and in between, I will discuss what problems I have faced; And why finally I gave it up.

If you are using Ubuntu or Linux Mint's latest operating system version then SNAP will be by default installed on your system and you can simply run the following installation command. But for other supported Linux systems you need to install the SNAPD, the steps can be found here in the SNAP installation documentation.

To start or initialize it after the installation, simply type:

in the command terminal.

Install Sosumi via SNAP


But after installing the above stable package of the Sosumi and later initializing it, I continuously got an error couldn't connect to KVM, although everything was working fine and tried all the rings and whistles but couldn't take it forward. Later, I removed the stable package and installed the developer one using the below command:

Yes, this time it did work and connected the KVM successfully but again with one more error.

So, I enable the xhost access control program by the following commands:

Once, it done, ran again sosumi command and this time I successfully able to start the process, first it downloaded the macOS installer image and then it triggered the Qemu machine with the Clover interface to install macOS on it.

Downloading macOS Catalina base system for Virtual machine

Few things which we should keep in mind before moving further to install macOS on Linux virtual machine.

  1. CPU should support hardware virtualization.
  2. Once up and running the VM could use more than 30 GB of storage on the physical hard drive, thus make sure you have enough space. You can check your Sosumi folder size at ~/snap/sosumi
  3. The script of Sosumi will automatically assign Virtual RAM, storage, CPU and other Virtual resources to macOS, if you want to customize that, it can be done in ~/snap/sosumi/common/launch
  4. The running of macOS could be slow.
  5. Even after spending hours, installing and setting up the macOS Catalina on Linux Qemu via this SNAP package, there is no surety it will work. Thus, this is just for experiment and incase it work fine, voilà enjoy it.

Clover Bootloader on Sosumi VM

If everything goes well soon you will see Clover bootloader, simply press the Enter button to start booting macOS.

Basu

Note: To release the mouse pointer or take out and to focus it on host operating system press CTRL + Alt + G simultaneously.

Sosumi Clover Qemu

macOS Utilities

If you are a user of macOS then you already know what to do next, however, if not then first we create a Disk partition to install on macOS on Linux VM. Select the Disk Utility option.

Apple Disk Partition

Select the first partition created by the Sosumi script which will be of 68.72 GB, enough to start with. Now, click on Erase button given in the top menu.

Format Disk in mas OS Extended (Journaled)

Simply give a name to the disk while leaving the rest of the options as it is, click on the Erase button.

Erase and name the partition Paint the sky golden mac os.

Once done close the Disk Utility and get back to the macOS Utilities screen.

Reinstall macOS

As here we are showing the system that we are not installing macOS from zero instead assume there is some problem and we want to reinstall a new copy of macOS, that's why we have the 'Reinstall macOS' option, so just select that and hit the Continue button.

Re-install macOS

Click the Agree button.

Select the Disk

The disk we have created above will show in this step, select that and hit the Install button to get the macOS on Linux Virtual Machine.

Select Hard drive

So, now if everything goes well it will install in around 50 minutes and if not then even I don't know how much time it would take because in my case, first, it showed 23 minutes than 11 hours.

It was kind of stuck here. I even went through the developer page of Sosumi but didn't find any relevant way to solve this problem, thus in my case I was not able to install it. I know running macOS on hardware and environment which is not meant for it is always a cumbersome job.

So, this was my predicament, however, if you have some time and want to experiment with it you can try Sosumi on your machine may be it work fine in your case. Nevertheless, I will also try the source of this snap package VM that is macOS-Simple-KVM, a set of tools to set up a quick macOS VM on QEMU, accelerated by KVM and will let you know my experience with it. Till then be safe and keep experimenting with Linux.

University Distinguished Prof Emeritus,
HHMI Investigator

Mac Os Catalina

Departments: Plant Biology - Plant, Soil & Microbial Sciences - Microbiology & Molecular Genetics
hes@msu.edu

Research: Molecular Biology of Plant-Pathogen Interactions

Plant diseases are a major cause of crop loss globally, representing a substantial obstacle in sustainable production of food and energy crops that are essential for basic human nutrition and health. Understanding how pathogens cause diseases therefore has broad implications in agriculture and human health. Sheng Yang He's group is studying plant-Pseudomonas syringae interactions to gain insights into some of the basic principles underlying bacterial pathogenesis and disease susceptibility in plants. To cause disease, P. syringae bacteria produce a variety of virulence factors, including numerous 'effector' proteins that are secreted through the type III protein secretion system (T3SS), and the phytotoxin coronatine, which functions as a molecular mimic of the plant hormone jasmonate.

Bacterial Effector Proteins

Our early work revealed the secretion function and part of the supramolecular structure of the T3SS of P. syringae. More recently, our work contributed to the discovery of two basic functions of P. syringae effector proteins: (i) suppression of plant immune responses and (ii) creation of an aqueous apoplast in which bacteria multiply in infected plant leaves (Figure 1). Over the years, we have studied a number of different P. syringae effectors (e.g., AvrPto, HopAO1, HopZ1, HopM1, AvrE, HopO1-1). Our current effort is directed at understanding how these various effectors contribute to disease development, with the hope that, one day, we could achieve the challenging goal of reconstituting disease susceptibility using host plant mutants that could recapitulate the collective virulence activities of P. syringae effectors.

The Immune Function of Plant Stomata

Plant stomata, formed by pairs of guard cells, are microscopic pores on the surface of all land plants. We found that plant stomata have an important immune function. Specifically, stomata close in response to plant and human pathogenic bacteria (Figure 2). Stomatal guard cells could perceive bacteria through pattern recognition receptors, such as flagellin receptor FLS2, activating a signaling cascade that requires the plant stress hormones salicylic acid. The signal transduction pathway underlying stomatal closure to pathogens is not well characterized. We are taking several approaches to increase our understanding in this area.

Jasmonate Signaling in Disease

For many years, we have been interested in identifying the host target of coronatine, a toxin produced by P. syringae. Coronatine shares striking structural similarities to the plant hormone jasmonate, which plays an important role in plant growth, development, and immunity, and counters stomatal and apoplastic defenses during bacterial pathogenesis. A few years ago, we used coronatine as a molecular probe to identify key regulators (e.g., JAZ repressors) of jasmonate signaling and components of the JAZ-COI1 jasmonate receptor complex (Figure 2). Our current work is aimed at achieving a deep understanding of the jasmonate signaling pathway, with the goal of modifying this pathway for enhanced pathogen resistance.

New Research Initiatives: The Next Phase of Study

Our current understanding of bacterial pathogenesis and disease susceptibility in plants remains largely one-dimensional, reflecting the heavy reliance on simplistic bilateral interactions of one pathogen and one host under static laboratory conditions. As a result, our knowledge of disease susceptibility does not accurately reflect the multi-dimensional features of plant disease development that occur in nature. To break new ground for the next phase of research on bacterial pathogenesis and disease susceptibility in plants, we have initiated two new projects:

Basu Bacteria Mac Os Download

  1. We are studying the molecular bases of the effects of temperature and humidity, which are known to significantly influence disease outbreaks in crop fields.
  2. We are developing a soil-based gnotobiotic plant growth system (called 'FlowPot') that enables the study of plant-microbe interactions in soil substrates, in the presence or absence of the endogenous microbiome. With further optimization, we hope that the FlowPot gnotobiotic system may be broadly useful in the study of interplay between the microbiome and plant biology.




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